Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO; Nobuaki NAKATSUKA; Takanari SHIGEMITSU; Kunisuke TANAKA; Shigeto MORITA; Shigeru SATOH; Takehiro MASUMURA

doi:10.1271/bbb.80337

This article has now been updated. Please use the final version.

Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO, Nobuaki NAKATSUKA, Takanari SHIGEMITSU, Kunisuke TANAKA, Shigeto MORITA, Shigeru SATOH, Takehiro MASUMURA

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Keywords: immunofluorescence microscopy, thin fresh-frozen section, rice grain, storage protein, prolamin

JOURNAL FREE ACCESS Advance online publication

Article ID: 80337

DOI https://doi.org/10.1271/bbb.80337

The final version of this article is now available: Vol. 72 (2008) , No. 10 p.2779

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Abstract

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

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