Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO; Nobuaki NAKATSUKA; Takanari SHIGEMITSU; Kunisuke TANAKA; Shigeto MORITA; Shigeru SATOH; Takehiro MASUMURA

doi:10.1271/bbb.80337

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Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains

Yuhi SAITO, Nobuaki NAKATSUKA, Takanari SHIGEMITSU, Kunisuke TANAKA, Shigeto MORITA, Shigeru SATOH, Takehiro MASUMURA

著者情報

キーワード: immunofluorescence microscopy, thin fresh-frozen section, rice grain, storage protein, prolamin

ジャーナルフリー早期公開

論文ID: 80337

DOI https://doi.org/10.1271/bbb.80337

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 10 p.2779

詳細

抄録

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

責任著者(Corresponding author)

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