Asymmetric Synthesis of (S)-α-Methylbenzylamine by Recombinant Escherichia coli Co-Expressing Omega-Transaminase and Acetolactate Synthase

Hyungdon YUN; Byung-Gee KIM

doi:10.1271/bbb.80410

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Asymmetric Synthesis of (S)-α-Methylbenzylamine by Recombinant Escherichia coli Co-Expressing Omega-Transaminase and Acetolactate Synthase

Hyungdon YUN, Byung-Gee KIM

著者情報

キーワード: acetolactate synthase, asymmetric synthesis, chiral amines, ω-transaminase

ジャーナルフリー早期公開

論文ID: 80410

DOI https://doi.org/10.1271/bbb.80410

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 11 p.3030

詳細

抄録

To produce (S)-α-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing ω-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-α-MBA produced in the ω-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.

責任著者(Corresponding author)

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