A Combination of Molecular Probe-Tandem Electrospray Ionization Mass Spectrometry: A Technique for Tracing Structural Changes in Phospholipid Hydroperoxides

Rumiko SHIMIZU; Ai NAGAI; Hiroko TOMINAGA; Megumi IMURA; Arnold N. ONYANGO; Minoru IZUMI; Shuhei NAKAJIMA; Shoichi TAHARA; Takao KANEKO; Naomichi BABA

doi:10.1271/bbb.80672

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A Combination of Molecular Probe-Tandem Electrospray Ionization Mass Spectrometry: A Technique for Tracing Structural Changes in Phospholipid Hydroperoxides

Rumiko SHIMIZU, Ai NAGAI, Hiroko TOMINAGA, Megumi IMURA, Arnold N. ONYANGO, Minoru IZUMI, Shuhei NAKAJIMA, Shoichi TAHARA, Takao KANEKO, Naomichi BABA

著者情報

キーワード: ESI MS, molecular probe, lipid hydroperoxide, tandem ESI MS, phosphatidylcholine hydroperoxide

ジャーナルフリー早期公開

論文ID: 80672

DOI https://doi.org/10.1271/bbb.80672

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 3 p.781

詳細

抄録

An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m⁄z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.

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