Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida

Francesca ANDREONI; Romina BOIANI; Giordano SERAFINI; Irene BIANCONI; Sabrina DOMINICI; Francesca GORINI; Mauro MAGNANI

doi:10.1271/bbb.80732

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Expression, Purification, and Characterization of the Recombinant Putative Periplasmic Hemin-Binding Protein (HutB) of Photobacterium damselae subsp. piscicida

Francesca ANDREONI, Romina BOIANI, Giordano SERAFINI, Irene BIANCONI, Sabrina DOMINICI, Francesca GORINI, Mauro MAGNANI

著者情報

キーワード: Photobacterium damselae, periplasmic hemin-binding protein (HutB), hemin binding protein, hemin, recombinant protein

ジャーナルフリー早期公開

論文ID: 80732

DOI https://doi.org/10.1271/bbb.80732

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 5 p.1180

詳細

抄録

HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (K_d) of 10 μM.

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