Purification and Characterization of an Extracellular Laccase from Phlebia radiata Strain BP-11-2 That Decolorizes Fungal Melanin

Shuji KANEKO; Minyi CHENG; Hiroshi MURAI; Shinji TAKENAKA; Shuichiro MURAKAMI; Kenji AOKI

doi:10.1271/bbb.80740

This article has now been updated. Please use the final version.

Purification and Characterization of an Extracellular Laccase from Phlebia radiata Strain BP-11-2 That Decolorizes Fungal Melanin

Shuji KANEKO, Minyi CHENG, Hiroshi MURAI, Shinji TAKENAKA, Shuichiro MURAKAMI, Kenji AOKI

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Keywords: fungal melanin, decolorization, Phlebia radiata, laccase, absorption spectrum

JOURNAL FREE ACCESS Advance online publication

Article ID: 80740

DOI https://doi.org/10.1271/bbb.80740

The final version of this article is now available: Vol. 73 (2009) , No. 4 p.939

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Abstract

Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH₂-terminal amino acid sequences.

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