Purification and Characterization of an Extracellular Laccase from Phlebia radiata Strain BP-11-2 That Decolorizes Fungal Melanin

Shuji KANEKO; Minyi CHENG; Hiroshi MURAI; Shinji TAKENAKA; Shuichiro MURAKAMI; Kenji AOKI

doi:10.1271/bbb.80740

Microbiology & Fermentation Technology Notes

Purification and Characterization of an Extracellular Laccase from Phlebia radiata Strain BP-11-2 That Decolorizes Fungal Melanin

Shuji KANEKO, Minyi CHENG, Hiroshi MURAI, Shinji TAKENAKA, Shuichiro MURAKAMI, Kenji AOKI

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Keywords: fungal melanin, decolorization, Phlebia radiata, laccase, absorption spectrum

JOURNAL FREE ACCESS

2009 Volume 73 Issue 4 Pages 939-942

DOI https://doi.org/10.1271/bbb.80740

View "Advance Publication" version (April 7, 2009).

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Abstract

Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH₂-terminal amino acid sequences.

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