Visualization of Aberrant Perinuclear Microtubule Aster Organization by Microtubule-Destabilizing Agents

Shinji SAKAUSHI; Kaori SENDA-MURATA; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.80754

This article has now been updated. Please use the final version.

Visualization of Aberrant Perinuclear Microtubule Aster Organization by Microtubule-Destabilizing Agents

Shinji SAKAUSHI, Kaori SENDA-MURATA, Shigenori OKA, Kenji SUGIMOTO

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Keywords: aster, microtubule, mitosis, microtubule organizing center (MTOC)

JOURNAL FREE ACCESS Advance online publication

Article ID: 80754

DOI https://doi.org/10.1271/bbb.80754

The final version of this article is now available: Vol. 73 (2009) , No. 5 p.1192

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Abstract

To analyze aberrant spindle formation by microtubule-targeting drugs, live cell imaging was performed using multi-fluorescent human MDA-MB-435 cells in which several spindle components were visualized. Time-lapse images revealed that nocodazole and vinblastine induced additional perinuclear asters at the onset of mitosis. These results imply that these drugs stimulate the microtubule-organizing activity, despite their microtubule-destabilizing properties.

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