Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening

Kosuke NAKAMURA; Masami INOUE; Megumi MAEDA; Ryohei NAKANO; Katsutoshi HOSOI; Kazuhito FUJIYAMA; Yoshinobu KIMURA

doi:10.1271/bbb.80889

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening

Kosuke NAKAMURA, Masami INOUE, Megumi MAEDA, Ryohei NAKANO, Katsutoshi HOSOI, Kazuhito FUJIYAMA, Yoshinobu KIMURA

著者情報

キーワード: endo-β-N-acetylglucosaminidase, free N-glycans, de-N-glycosylation, tomato fruit ripening, Lycopersicon esculentum

ジャーナルフリー早期公開

論文ID: 80889

DOI https://doi.org/10.1271/bbb.80889

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 2 p.461

詳細

抄録

In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the high-mannose type N-glycans with the Manα1-2Manα1-3Manβ1-4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）