Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana

Yasuo KATO; Kazuaki SHOJI; Makoto UBUKATA; Kengo SHIGETOMI; Yukio SATO; Noriyuki NAKAJIMA; Shinjiro OGITA

doi:10.1271/bbb.90226

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Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana

Yasuo KATO, Kazuaki SHOJI, Makoto UBUKATA, Kengo SHIGETOMI, Yukio SATO, Noriyuki NAKAJIMA, Shinjiro OGITA

著者情報

キーワード: tulip, tuliposide, tulipalin, enzyme purification, Tulipa gesneriana

ジャーナルフリー早期公開

論文ID: 90226

DOI https://doi.org/10.1271/bbb.90226

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 8 p.1895

詳細

抄録

An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.

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