Further Evaluation of the Localization and Functionality of Hemagglutinin Epitope- and Fluorescent Protein-Tagged AtMinD1 in Arabidopsis thaliana

Makoto T. FUJIWARA; Dongliang LI; Yusuke KAZAMA; Tomoko ABE; Tomohide UNO; Hiroshi YAMAGATA; Kengo KANAMARU; Ryuuichi D. ITOH

doi:10.1271/bbb.90309

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Further Evaluation of the Localization and Functionality of Hemagglutinin Epitope- and Fluorescent Protein-Tagged AtMinD1 in Arabidopsis thaliana

Makoto T. FUJIWARA, Dongliang LI, Yusuke KAZAMA, Tomoko ABE, Tomohide UNO, Hiroshi YAMAGATA, Kengo KANAMARU, Ryuuichi D. ITOH

著者情報

キーワード: Arabidopsis thaliana, arc11, chloroplast division, plastid division

ジャーナルフリー早期公開
電子付録

論文ID: 90309

DOI https://doi.org/10.1271/bbb.90309

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 7 p.1693

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抄録

Symmetric chloroplast division requires a prokaryote-derived division regulator protein MinD, whose subchloroplastic localization remains to be completely established. We investigated the localization and functionality of AtMinD1 (Arabidopsis thaliana MinD) fused with a dual hemagglutinin epitope (dHA) or a yellow fluorescent protein (YFP). AtMinD1-dHA, which successfully complemented the arc11/atminD1 mutant phenotype, was predominantly located at the envelope membrane and the mid-chloroplast constriction site. Meanwhile, AtMinD1-YFP was non-functional and showed suborganellar localization partly similar to that of AtMinD1-dHA. This prompts us to reevaluate earlier transgenic and transient expression studies using fluorescent protein-tagged AtMinD1.

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