Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease

Shirou TSUCHIDA; Takashi SHIGEMATSU; Taemi YAHARA; Kana NUNOME; Naoya HAMAUE; Takashi AOKI

doi:10.1271/bbb.90822

This article has now been updated. Please use the final version.

Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease

Shirou TSUCHIDA, Takashi SHIGEMATSU, Taemi YAHARA, Kana NUNOME, Naoya HAMAUE, Takashi AOKI

Author information

Keywords: protease, substrate specificity, homogeneous assay, green fluorescent protein, tobacco etch virus protease

JOURNAL FREE ACCESS Advance online publication

Article ID: 90822

DOI https://doi.org/10.1271/bbb.90822

The final version of this article is now available: Vol. 74 (2010) , No. 4 p.869

Details

Abstract

The utility of the homogeneous assay for fluorescence concentrated on membrane (HAFCOM) in the analysis of the substrate specificity of protease was investigated using tobacco etch virus (TEV) protease. The V_max of TEV protease against variants of a substrate was obtained by a simple procedure. It was considered that HAFCOM was more accurate than other endpoint measurements of protease assay.

Corresponding author

Register with J-STAGE for free!