We purified the catalases of both the spinach leaves and rice-plant leaves by the n-butanol treatment and isolated them into crystalline forms. The absorption spectrum of the spinach leaf catalase is identical with that of the animal catalase. The activity of the spinach leaf catalase has its optimum of 15°, but that of the rice-plant leaf catalase at 35° These optimum temperatures have resemblance to the circumstances where they grow and are independent of enzyme purity.
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