Abstract
An improved method is described for separating saturated fatty acids by reversed-phase paper chromatography. The 2, 4-dinitrophenylhydrazides prepared from saturated fatty acids from C2 to C22 are run upward on paper impregnated with tetralin, using 90% methanol-acetic acid-tetralin, 80% ethanol-acetic acid-tetralin, or 90% methanol-tetralin as the moving solvent. The simultaneous separations of all even-carbon acids from C6 to C22, all odd-carbon acids from C7 to C19, and also all odd- and even-carbon acids from C7 to C19 can be successfully performed by means of this paper chromatography. The method is useful for the detection of component saturated fatty acids in natural fats.
