Bulletin of the Agricultural Chemical Society of Japan
Online ISSN : 1881-1272
Print ISSN : 0375-8397
ISSN-L : 0375-8397
A Micro Amperometric Titration of Thiol-Group in Plant Tissues by the Use of a Vibrating Platinum Electrode
Yasutoyo NAGAITetsuro KÔNOSaburo FUNAHASHI
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1957 Volume 21 Issue 2 Pages 121-125

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Abstract
A method for the micro amperometric determination of thiol-groups as small as from 0.1 to 1.0μ mole, corresponding to 10-100μg of cysteine, was devised. The thiol-group was titrated in ammoniacal solution in the stream of nitrogen with silver nitrate solution, using an ultramicroburette. The course of the titration and the end point was detected amper-ometrically using platinum vibrating electrode. The method was suitable for the determina-tion of a minute amount of thiol-groups, especially those found in plant tissues, which used to contain such substances as might otherwise disturb the determination. Contents of acid-soluble thiol compounds in etiolated mung bean seedlings at various stages of growth were determined by the method described here.
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