Electrolytic Reduction of Physiologically Important Substances at Controlled Potential

Part I. A Partially Active Product of Diphosphopyridine Nucleotide

Abstract

By means of the electrolytic reduction of diphosphopyridine nucleotide at controlled potential using mercury as the cathode, the author has obtained a product which showed an absorption spectra similar to that of dihydrodiphosphopyridine nucleotide. The optical density of the product at 340mμ diminished partly with the addition of alcohol-dehydrogenase and acetaldehyde or with that of malic acid-dehydrogenase and oxaloacetate as the oxidizing system. The same absorption band was completely disappeared by the addition of a fraction obtained from mung bean seedlings.