Bulletin of the Agricultural Chemical Society of Japan
Online ISSN : 1881-1272
Print ISSN : 0375-8397
ISSN-L : 0375-8397
Column Chromatographic Fractionation of Apricot Emulsin
Akira BABA
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1957 Volume 21 Issue 3 Pages 166-172

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Abstract
Crude emulsin of apricot (Prunas armenica) kernel was prepared by the method of tannin-fractionation. It was then purified by a fractional ammonium sulfate precipitation. The purified enzyme was further fractioned by adsorbing the enzyme on a CM-cellulose column and eluting it with the diluted Mcllvaine's buffer solution. By this chromatography, six peaks of activities of β-glucosidase and β-xylosidase were developed. From one component of these peaks, petal-like crystals were obtained. The fractions thus obtained by chromato-graphic fractionation and crystallization were found differ with regard to the ratio of the β-glucosidase activity to the β-xylosidase one.
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