抄録
The inducing action of lactic acid on the formation of racemiase was investigated in various aspects. Calcium carbonate revealed remarkable effect on the formation of racemiase, when lactate was not added as an inductor. Characteristic time course of formation of both lactic acid and racemiase in culture of Cl. acetobutylicum was pursued. Pyruvate was capable or promating racemiase activity to some extent in iron-deficient culture. Lactic acid was produced extraordinarily in the presence of cyanide of bicarbonate, but the formation of racemiase was not affected significantly. With intact cellss or Cl. acetobutylicum in adequate conditions, homo-lactic acid fermentation was realized, namely, ninety-seven per cent of glucose consumed was converted to racemic lactic acid. Details of paper chromatographic technique in separating racemic and optically active zinc lactates were presented.
