Studies on the Chitinolytic Enzymes of Black-koji Mold Part I.

Viscometric Determination of Chitinase Activity by Application of Glycol Chitin as a New Substrate

抄録

It has been found that glycol chitin is a suitable substrate for the viscometric determination of chitinase activity, because the viscosity of its aqueous solution is not affected by the presence of added salt and the changes of pH, differing from chitosan acetate and carboxymethyl chitin used by earlier workers. Using this substrate the viscometric activity is determined, basing on the observation that the time required to halve the viscosity of reaction mixture is inversely proportional to the amount of enzyme used.