Studies on Osmophilic Yeasts

Part XIV. Partial Purification of Polyol Dehydrogeuase from Pichia miso and the Properties of this Enzymes

Abstract

In order to ascertain the existence of polyol dehydrogenase which seemed to take a part in polyalcohol production by Pichia miso, the preparation and the partial purification of polyol dehydrogenase from thecells of Pichia miso were carried out. Some properties of this enzyme preparation and the identification of the products formed by this enzyme action were also described. This enzyme preparation was found to catalyze the following reactions:
D-arabitol+DPN⁺⇔D-Xylulose+DPNH+H⁺, Polyalcohol+DPN⁺⇔Ketose+DPNH+H⁺.