Studies on Pectolytic Enzymes of Molds

Part V. The Fractionation of Pectolytic Enzymes of Coniothyrium diplodiella (1)

Abstract

Experiments have been made on fractionation of the pectolytic enzymes produced by Coniothyrium diplodiella. It has been observed that 30 to 35% of the polygalacturonase (PG) activity of the pectolytic enzymes of the said microorganism is salted out with ammonium sulfate, and this portion contains endo-PG I, endo-PG II and pectin esterase (PE)(with a trace of exo-PG). The endo-PG I accounts for 60 to 65% of the total PG activity, and the endo-PG II, 25 to 30%. Both types of endo-PG scarcely act on pectin, and hydrolyze pectic acid to the extent of 65 to 70%.