Abstract
Of the pectolytic enzyme produced by Coniothyrium diplodiella, examination has been made on the enzymatic composition of the portion which is not salted out with saturated ammonium sulfate. This portion accounts for 40 to 50% of the total polygalacturonase (PG) activity of such enzymes, and treatment with rivanol and chromatography on Duolite CS-101 and DEAE-cellulose disclosed the presence of two types, endo-PG III and exo-PG, which occupy 60 to 65% and 28 to 30% of the PG activity respectively. Endo-PG III hardly acts on pectin and hydrolyzes pectic acid by no more than 35 to 40% of the total glycosidic bonds. Exo-PG also acts on pectic acid and hydrolyzes it to the extent of 100%.
