Bacterial Synthesis of Nucleotides

Part X. Phosphorylation of 5-Amino-4-imidazole Carboxamide Riboside

抄録

5'-Phosphoribosyl 5-amino-4-imidazole carboxamide was synthesized in a reaction mixture containing 5-amino-4-imidazolecarboxa-mide riboside, p-nitrophenylphosphate, and the bacteria characterized to phosphorylate at C_5' just as the nucleosides were phosphorylated. This phosphorylated material was isolated chromatographically and identified chemically or enzymatically. The other bacteria syn-thesizing 3'-or 2'-nucleotides also phosphory-lated a little probably at C_{3' (or 2')}.