Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
29 巻, 12 号
選択された号の論文の26件中1~26を表示しています
  • Part VI. Oxidation of Thiosulfate to Tetrathionate by T. thiooxidans
    Masayo OKUZUMI, Yasou KITA
    1965 年 29 巻 12 号 p. 1063-1068
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    In the oxidation of thiosulfate at pH 4.5 tetrathionate was formed as an intermediate, and the thiosulfate-oxidizing enzyme was active in acidic pH range in contrast to the enzyme of T. thioparus and Thiobacillus X.
    Phosphate did not seem to affect the oxidation of thiosulfate but rather affect the conversion of tetrathionate. In the absence of phosphate, tetrathionate, which was produced from thiosulfate oxidation, seemed to accumulate without undergoing further conversion.
  • Part VII. Metabolism of Tetrathionate by T. thiooxidans
    Masayo OKUZUMI
    1965 年 29 巻 12 号 p. 1069-1073
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    Quantitative oxidation of tetrathionate to sulfate was achieved with freshly harvested cells of T. thiooxidans; pH optimum for the oxidation of tetrathionate by the washed cells was 2_??_3, and the activity fell markedly at pH above 3.5.
    Tetrathionate might be enzymatically dismuted to pentathionate and trithionate under anaerobic conditions with crude extracts of T. thiooxidans; pH optimum for the reaction was about 2.7 and the activity fell strikingly at pH 4.7. The formed trithionate might be further hydrolyzed to thiosulfate and sulfate.
  • Part VI. The Heat Destruction of Amino Acids in Deffatted Soybean Flour
    Harue TAIRA, Hirokadzu TAIRA, Kei-ichiroh SUGIMURA, Yosito SAKURAI
    1965 年 29 巻 12 号 p. 1074-1079
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    The influence of heat treatment on defatted soybean flour was studied. The eighteen kinds of amino acids were determined by microbiological assay method.
    The heat destruction of the amino acids was found to occur when defatted soybean flour was autoclaved. At0Kg/cm2 (100°C) for one, two, and four hours, no remarkable destruction was observed on the amino acids. On the other hand, cystine, lysine and arginine were destroyed under following heat conditions at 0.35Kg/cm2 (108°C), 0.7Kg/cm2 (115°C) and 1.4Kg/cm2 (126°C). Especially, heating at 1.4Kg/cm2 (126°C) for four hours, a large amount of cystine, lysine and arginine and a small amount of tryptophan and serine were destroyed, but all other amino acids were not destroyed by any heat treatment.
    The different types of heat destruction of cystine, lysine and arginine were observed when defatted soybean flour was autoclaved under the systematically specified heating conditions.
  • Part VII. The Influence of Added Water on the Heat Destruction of Amino Acid in Defatted Soybean Flour
    Harue TAIRA, Hirokadzu TAIRA, Kei-ichiroh SUGIMURA, Yosito SAKURAI
    1965 年 29 巻 12 号 p. 1080-1083
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    The influences of the added water, the period of heating and the temperature on destruction of amino acids of defatted soybean flour were studied. The eighteen amino acids available to the microbiological assay using lactic acid bacteria were assayed with respect to those products treated under different heating conditions in the presence of water 3 and 6 times the weight of the soybean flour, and in the absence of water under the pressure of 0.7Kg/cm2 (115°C) and 1.4Kg/cm2 (126°C) for various heating periods.
    Amino acids except for lysine, arginine, cystine, tryptophan and serine were not destroyed in any heat treatment examined. The destruction of lysine and arginine was mainly due to the conditions of the amount of the added water, and those of cystine, tryptophan and serine were chiefly due to the period and the temperature of heat treat-ment.
  • Bunji MARUO, Toshie HATTORI, Hajime TAKAHASHI
    1965 年 29 巻 12 号 p. 1084-1089
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    Among 8 strains of algae grown with C14O2 as a sole source of carbon, two species of Trebouxia produced appreciable amounts of two photosynthetic products in the culture medium. One of them was identified as sucrose by cochromatography and by acid hydrolysis. The other compound was identified as ribitol by paper chromatography, paper electrophoresis, periodic acid oxidation, recrystallization with authentic ribitol and finally by the enzymatic method with ribitol dehydrogenase.
  • Part I. Synthesis of Piperitenone by the Condensation of Mesityloxide with 4-Diethylaminobutanone-(2)
    Hiroo UEDA, Ken'ichi TAKEO, Ping-Li TSAI, Chuji TATSUMI
    1965 年 29 巻 12 号 p. 1090-1098
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    The condensation of mesityloxide with 4-diethylaminobutanone-(2) in the presence of potassium t-butoxide gave the products containing piperitenone together with isoxylitones, which were identified by gas chromatography and chemical methods. The effects of the combination of condensing agent, solvent, molar ratio of reagents and reaction temperature on the components in the condensation products were studied. It was found that piperitenone was able to obtain in an yield of 51% on the basis of 4-diethylaminobutanone-(2) by the use of Triton-B as a condensing agent. Also piperitenone can be isolated from the condensation products by the treatment with semicarbazide.
  • Part II. The Effect of Frozen Storage on the Milk Casein
    Takeo NAKANISHI, Takatoshi ITOH
    1965 年 29 巻 12 号 p. 1099-1103
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    A study has been made with the changes occurred in the milk casein during frozen storage. The flocculated protein formed during storage of frozen milk was resolved in 4_??_5 peaks in free-boundary electrophoretic pattern, using veronal buffer containing 7M urea.
    The flocculated protein was mainly casein. It may be considered that portions of casein have undergone some modification or denaturation during the destabilization process.
    Significant increase has occurred in the relative proportion of β-casein in the casein remaining in supernatant after removal of flocculate, indicating that the partial fractionation of casein component occurred as a result of flocculation.
  • Part X. Phosphorylation of 5-Amino-4-imidazole Carboxamide Riboside
    Koji MITSUGI, Shinji OKUMURA, Teruo SHIRO, Masahiro TAKAHASHI
    1965 年 29 巻 12 号 p. 1104-1108
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    5'-Phosphoribosyl 5-amino-4-imidazole carboxamide was synthesized in a reaction mixture containing 5-amino-4-imidazolecarboxa-mide riboside, p-nitrophenylphosphate, and the bacteria characterized to phosphorylate at C5' just as the nucleosides were phosphorylated. This phosphorylated material was isolated chromatographically and identified chemically or enzymatically. The other bacteria syn-thesizing 3'-or 2'-nucleotides also phosphory-lated a little probably at C3' (or 2').
  • Part XI. Phosphoryl Transfer between Mononucleotides and Nucleosides via A Phosphotransferase Reaction
    Koji MITSUGI, Akira KAMIMURA, Michiko KIMURA, Shinji OKUMURA
    1965 年 29 巻 12 号 p. 1109-1118
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    The optimal pH of the phosphoryl transfer reaction between nucleotide and nucleoside was found to be not always the same. In the reaction mixture of Ps. trifolii, the optimal pH of its activity was at around 8.5, and 5'-nucleotide was the most excellent donor. Phophotransferase activity of Prot. mirabilis had an optimal pH at around 5.0, and 2'- and 3'-nucleotide were observed to just make a donor.
    These donor- and product-isomer specificities of both groups of bacteria were evident to be invariable, regardless of reaction pH and the cultural conditions of each bacterium, and, therefore, are considered to be attributed to their substantial characters.
    The fact that the phosphoryl transfer reaction between purine nucleosides and pyrimidine nucleotides was reversible suggests that this enzymatic activity may catalyze the phos-phoryl interconversion of 5'-nucleotides in these bacterial cells of A group.
    Cupric ions and arsenate were observed to stimulate the synthesis of nucleotide.
  • Part III. Thioketo-Enethiol Tautomerism of β-Aryl-α-thiopyruvic Acids and Geometrical Configuration of 4-Arylidene-1, 3-oxathiolan-5-ones
    Motohiro NISHIO, Teiichiro ITO
    1965 年 29 巻 12 号 p. 1119-1122
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    Measurements of NMR spectra of β-aryl-α-thiopyruvic acids revealed that they exist dominantly in the form of trans cinnamic acid configuration. Geometrical configuration of 4-grylidene-1, 3-oxathiolan-5-one was also determined to be trans in regard to aryl and carbonyl groups.
  • Part V Partial Purification and Properties of the Enzyme from Aerobacter cloacae
    Nobuzo TSUMURA, Tomotaro SATO
    1965 年 29 巻 12 号 p. 1123-1128
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    Glucose isomerizing enzyme was partially purified after investigation on the properties of crude enzyme extract. The crude extract was partly inactivated by the contact with air. The addition of manganese was effective to improve the stability. Magnesium was essential to the enzyme action and cobalt accelerated the reaction.
    The maximal activity was observed at pH about 7.6 and 50°C was optimal for the incubation time of 30 minutes. The enzyme solution reacted with D-xylose as well as D-glucose. The activity of the enzyme was inhibited at high glucose concentrations.
  • Part VI. Properties of the Enzyme from Streptomyces phaeochromogenus
    Nobuzo TSUMURA, Tomotaro SATO
    1965 年 29 巻 12 号 p. 1129-1134
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    An enzyme which catalyzes the conversion of D-glucose to D-fructose has been demonstrated in cell-free extracts of Streptomyces phaeochromogenus grown in the presence of D-xylose. The enzyme preparation reacts with D-glucose and D-xylose, but not with other sugars tested. It appears to require magnesium for the maximal activity and the addition of cobaltous ion remarkably intensifies the heat tolerance of the enzyme. The maximal activity occurs at about pH 9.3_??_9.5. Equilibrium is reached when about 52% fructose is present in the reaction mixture. The enzyme has half-maximal activity when the concentration of D-glucose is about 0.3M at pH 9 and 60°C.
  • Part I. Some Characteristices of the Twelve Phages Obtained from the Abnormally Fermented Broths
    Motoyoshi HONGO, Akira MURATA
    1965 年 29 巻 12 号 p. 1135-1139
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    Some characteristics of the twelve phages were given. Phages were obtained from the twelve abnormal broths in the industrial production of acetone and butanol by use of Cl. saccharoperbutylacetonicum, producing a high proportion of butanol. These new phages were designated as HM and numbered consecutively in the order of appearance. The HM-phages were highly specific for the strains of this bacterium. Each phage could be distinguished from the others by its differential host specificity against various phage-resistant mutants of this bacterium. The HM-phages were not temperate ones under our experimental conditions. They were divided into two groups on the basis of their stability in the salts solution. While one group was slightly less stable in 0.85 percent saline or 0.067M phosphate buffer, the other was strikingly unstable. The addition of magnesium ion was effective for increase in stability of both groups.
  • Part II. Enumeration of Phages by the Application of the Plaque-Count Technique and some Factors Influencing the Plaque Formation
    Motoyoshi HONGO, Akira MURATA
    1965 年 29 巻 12 号 p. 1140-1145
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
    The double-layer method similar to that described by Adams, with modifications to incubate the plates anaerobically, was applied to assay the phages of butanol-producing bacteria, Cl. saccharoperbutylacetonicum, after the studies on some factors influencing the plaque formation. Factors influencing the number and size of plaque were as follows; agar concentration and amount of overlayer medium, pH of media, age and number of host cells, temperature and period of incubation, and so on. Plaques formed by this method were medial size and easily counted. Assay of viable cells of this anaerobic bacterium was also possible by this method with slight modifications.
  • Yorinao INOUE, Tsuneji SUTOU, Teijiro UEMURA
    1965 年 29 巻 12 号 p. 1146-1147
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • Hideaki YAMADA, Osao ADACHI, Koichi OGATA
    1965 年 29 巻 12 号 p. 1148-1149
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • Masao FUJIMOTO, Kazuo UCHIDA
    1965 年 29 巻 12 号 p. 1150-1151
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2a
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2b
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2c
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2d
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2e
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e2f
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e3a
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e3b
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
  • 1965 年 29 巻 12 号 p. e3c
    発行日: 1965年
    公開日: 2008/11/27
    ジャーナル フリー
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