Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Defluorination of Monofluoroacetate by Bacteria
Part I. Isolation of Bacteria and their Activity of Defluorination
Kenzo TONOMURAFusae FUTAIOsamu TANABETakashi YAMAOKA
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1965 年 29 巻 2 号 p. 124-128

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(1) Bacteria capable of assimilating FA as a source of carbon have been isolated from soil obtained at the factory producing FA. The assimilation of FA was fairly stable and very strong in this organism. It appeared to belong to a member of Pseudomonas species.
(2) The enzyme that catalyzes defluorina-tion of FA to form glycolic acid and fluoride ions was induced, when bacteria were grown in a defined salt medium containing FA as a sole source of carbon. Glycolic acid was identified as a salt of S-benzylthiuronium after being isolated by a chromatography in silica colum.
(3) The liberation of fluoride ions from monofluoroacetamide and monofluoropropiona-te was very small compared with the libera-tion from FA. Monochloro-, monobromo-, and monoiodoacetate were not dehalogenated by the enzyme.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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