Production of Nucleic Acid-Related Substances by Fermentative Processes

Part IX. Chemicals-Resistant Mutants of an Inosinic Acid-Producing Strain of Micrococcus glutamicus

Abstract

The inhibitory effects of 20 compounds including purine analogues on the growth of Micrococcus glutamicus No. 534-348, an inosinic acid-producing adenine-auxotroph, and No. 534, a prototrophic strain, were investigated.
A number of strains resistant to each of 6-mercaptoguanine, 6-thioguanine, 8-aza-guanine, mitomycin C and sulfanilamide were induced from strain No. 534-348, and their inosinic acid productivities were compared with their parent strain. Among them, MGR-25, a 6-mercaptoguanine-resistant strain, accumulated more inosinic acid than its parent strain. Furthermore, the strain MGR-25 was differentiated in its morphology, frequency of spontaneous reversion to prototrophic type in adenine-deficient medium, and the effectiveness of hypoxanthine to increase the inosinic acid accumulation.