(1) The microbiological method for the separation by paper chromatography and the determination of deoxyribosides with
Lacto-bacillus leichmannii 7830 as a test organism was devised. The method described in this paper is very specific, and has high sensitivity for deoxyribosides, and it is possible to determine 100_??_500mμg, namely 1_??_2×10
-9 mole levels of deoxyribosides per 5ml medium., It is found that all fine deoxyribosides tested have the same growth-promoting. effect. The recoveries ranging between 95 and 105% were obtained when the authentic deoxyribosides were separated by paper chromatography and estimated with the organism.
(2) The, growth factors for
L. leichmannii in the extract of 13 days old chick embryo treated for 60 minutes at pH 12_??_13 and 120°C, so-called alkalii stable factors, were identified by paper chromatographic separa-tion with the cysteine-sulfuric acid reagent and microbiologically. So-called alkali stable factors were deoxyribosides, i.e., deoxycytidine, deoxyuridine and thymidine, and unidentified compound (temporarily peak 1).
(3) The change of each constituent of alkali stable factors of the embryo with the incuba-tion was estimated by the method presented in this paper. The ratios of thymidine and deoxyuridine to total alkali stable factors in-creased gradually with the incubation until the 15th day incubation. Peak 1 compound, on the contrary, began to decrease from about the 5th day and attained to minimum at the 15th day in incubation. The change of ratio of deoxycytidine showed the tendency of the increase from the earlyy stages until the in-cubation and attained to the maximum value at the 12th day. And then, after the ratio once decreased until the 14_??_15th day incubation, again the ratio of deoxycytidine increased gradually.
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