Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Intracellular Peptidase of Bac. subtilis
Noshi MINAMIURATakehiko YAMAMOTOJuichiro FUKUMOTO
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1966 年 30 巻 2 号 p. 186-192

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A peptidase was isolated from the cells of amylase-producing Bac. subtilis by means of cell lysis with egg white lysozyme, followed by freezing and thawing, salting out, dialysis and ion-exchanger column chromatography. The enzyme required manganese ion to show the enzyme activity. Also the enzyme was stable in the presence of magnesium ion. The enzyme hydrolyzed various synthetic peptides by stepwise removal of the amino terminal amino acid of peptides and thus the peptidase was found to be aminonentidase.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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