Studies on the Amino Acid Sequence of Holmes Rib-Grass Strain Virus Protein

Part I. Separation of pH 4.5 Soluble Tryptic Peptides and Amino Acid Sequences of Some Small Peptides of Holmes Rib-Grass Strain Virus Protein

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The pH 4.5 soluble tryptic peptides of Holmes Rib-Grass strain virus protein were fractionated by Dowex 1×2 column chromatography and their amino acid compositions were determined. The purification of the peptides has been achieved by chromatography on Dowex-l×2 following paper chromatography. The sequences of five small peptides have been established. These are as follows. T-3; Asn-Arg, T-5; Asp-Thr-Val-Arg, T-6; Ser-Phe-Asp-Thr-Arg, T-7; Phe-Pro-Asp-Thr-Gly-Phe-Arg, T-9; Val-Asp-Asp-Ala-Thr-Val-Ala-Ileu-Arg.