Bacteriophages of Clostridium saccharoperbutylacetonicum

Part VIII. Inhibition of HM-Phages by Antibiotics

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The action of the eighteen antibiotics on the growth of phage HM 2 of Clostridium saccharoperbutylacetonicum was studied by means of one-step growth technique as a part of basic study for preventing phage-infection in industrial fermentation. Three antibiotics (even 100μg/ml) had no effect upon the growth of phages and bacteria. Fourteen antibiotics were effective against both, and the effect of these antibiotics on the phage growth closely paralleled that on bacterial growth.
Of the antibiotics tested, only chloramphenicol (CP) showed selective inhibition of phage growth, i.e., CP (ca. 1μ/ml) inhibited completely the phage growth, whereas it (up to 100μg/ml) failed to inhibit the growth of uninfected host bacteria. No phage yield occurred even when CP was added late in the latent period (within 40min.). Addition of CP after that time resulted in inhibition of further phage growth. CP had no direct phagicidal action, did not prevent adsorption and injection and did not inhibit lysis of infected cells. When CP was added in any time of latent period, protein synthesis in infected cells was stopped immediately, whereas total DNA continued to rise but at a reduced rate. These results indicate that CP inhibits the intracellular phage growth by suppressing the protein synthesis in infected cells.
CP inhibited also the growth of elevent HM-phages other than HM 2.