Abstract
Some physicochemical properties and amino acid composition of alkaline proteinase from Aspergillus sojae were found to be as follows: The isoelectric point was at pH 5.1. The molecular weight was 25500 using the Sheraga-Mandelkern's formula, based upon the values of the sedimentation coefficient (s_??_20, w=2.82 S), the intrinsic viscosity ([η]=0.027 dl/g), and the partial specific volume (_??_=0.726ml/g). The enzyme contains 16.8% of nitrogen and is composed of 250 residues of amino acid; Asp31, Glu19, G1y27, Ala32, Val18, Leu14, Ile14, Ser28, Thr18, (_??_)1, Met2, Pro6, Phe7, Tyr8, Trp2, His5, Lys14, Arg3, (amide-NH3)20.
