Physiological Studies on Thiobacilli

Part III. The Oxidation of Thiosulfate by Particulate Fraction of Thiobacillus thiooxidans

Abstract

Thiosulfate was oxidized to tetrathionate and an unknown oxidized sulfur compound. The activity was found in the particulate fraction (a precipitate obtained by centrifuging (at 90, 000 ×g for 60min) the supernatant of centrifugation at 10, 000 ×g for 30min). Treatment with deoxycholate caused inactivation of the enzyme. Considering from these points and electronmicrograph, the enzyme would be located on the cell membrane.