Bacteriophages of L-Glutamic Acid-Producing Bacteria

Part IX. Properties of Deoxyribonucleic Acids from Brevibacterium Phages and Their Host

抄録

Deoxyribonucleic acids were isolated from Breribacterium lactofermentum No. 2256 and its four representative phages belonging to different serological groups, i.e., P465 (group I), P 468 II (group II), Ap 85 III (group III) and P 4 (group IV), by phenol extraction.
DNA's isolated from the four phages contained only usual four bases, i.e., guanine, adenine, cytosine and thymine. The G-C content of the phage DNA was determined by thermal denaturation method (T_m); the values of P 465, P 468 II, Ap 85 III and P 4 were 54.0, 54.6, 56.6 and 55.3%, respectively. Sedimentation coefficient was measured by ultracentrifugal analysis using ultraviolet optics; _{s20, w} of the phage DNA's were 15.5 to 31.8s.
Unusual bases were not detected also in the host bacterial DNA. The G-C content of the bacterial DNA determined by paperchromatography was 55.1% which came very close to the G-C content derived from the T_m.