Abstract
As a counterwork for preventing phage growth in industrial L-glutamic acid fermen-tation, the screening of effective chemical agents was carried out. Of the sixty chemicals such as surface-active agents, antibiotics, organic acids, dyestuffs and others, citrate, oxalate and tri- or tetrapolyphosphate showed the selective inhibition for phage growth. The concentrations of these chemicals required to inhibit phage growth were 5×10-2M for citrate or oxalate, and 0.l% for tri- or tetrapolyphosphate, respectively.
Application of these selected chemicals to L-glutamic acid fermentation resulted in the complete inhibition of phage growth without affecting on L-glutamic acid production. These results show that the addition of citrate, oxalate, sodium tri- or tetrapolyphosphate to the medium is a promising mean for preventing phage infection in the plant.
