Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Enzymatic Determination of L-Phenylalanine and L-Tyrosine
Kazuko UCHIYAMAHideaki YAMADATatsurokuro TOCHIKURAKoichi OGATA
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1968 年 32 巻 6 号 p. 764-768

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An enzymatic method for the determination of phenylalanine and tyrosine has been described. This method is based on the formation of cinnamic acid from phenylalanine or the formation of p-coumaric acid from tyrosine by phenylalanine ammonialyase of Rhodotorula. Cinnamic acid and p-coumaric acid, which are formed in stoichiometric amounts, are determined spectrophotometrically. Other amino acids and D-isomers of phenylalanine and tyrosine have no effect on this determination.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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