Accumulation of 6, 7-Dimethyl-8-Ribityllumazine by Auxotrophic Mutant of Bacillus subtilis

Abstract

One of adenine-riboflavin doubleless mutants derived from Bacillus subtilis strain AJ 1987, an adenine requiring and inosine producing strain, was found to accumulate a fluorescent material in the culture medium. This material was identified as 6, 7-dimethyl-8-ribityl-lumazine (LUM). Several culture conditions for LUM formation were studied. In general, accumulation of LUM reached approximately 90mg per 100-ml medium in 3 days.