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Part XIII. Properties of HM 2 Phage Inactivated by DNase and the Tail of HM 2 Phage
Motoyoshi HONGO, Seiya OGATA
1969 Volume 33 Issue 1 Pages
1-9
Published: 1969
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To understand the mechanism of inactivation of HM 2 phage by DNase, studies were made on the properties of the inactivated phage, the structure of the intact and inactivated phages and the properties of the tail.
HM 2 phage inactivated by DNase still retained the unique and large protein structure which combined fully with anti-HM 2 phage serum. According to electron microscopic observation, DNase-inactivated HM 2 phage looked empty and retained its tail surrounded with appendages. Ultracentrifugal analysis indicated that DNase-inactivated phage became a low molecular weight material after DNA had been extruded. The tail of the ghost retained normal ability to attach to its host bacteria,
Cl. saccharoperbutylacetonicum Nl-4.
The tail of HM 2 phage does not have killing and lysing ability.
From these results, it is concluded that the inactivation of HM 2 phage by DNase is not due to the damage of the coat protein and tail structure, but due to the damage of DNA itself.
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Part II. Isolation and Purification of Cytochrome c and a Blue Protein from Rice Bran
Shoji IDA, Yuhie MORITA
1969 Volume 33 Issue 1 Pages
10-17
Published: 1969
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A procedure developed for the isolation and purification of soluble hemoproteins from rice embryo has been applied for the same purpose for rice bran. The method involved extraction with dilute phosphate buffer, fractionation with ammonium sulfate, ion-exchange chromatography on CM-Sephadex C-50, and gel filtration chromatography on Sephadex G-75. Cytochrome c and a blue protein were obtained as the major soluble basic proteins. Rice bran cytochrome c was crystallized from ammonium sulfate solution. The absorption spectrum and other physico-chemical properties were found to be identical with those of rice embryo cytochrome c as reported in the previous paper. The blue protein was obtained in a highly purified form. The absorption spectrum of the oxidized form shows major bands at 280 and 600 mμ with a hump around 450 mμ The blue protein was reduced by dithionite, ascorbate, hydroquinone with a concominant disappearance of its color, but the reduced form could not be oxidized by the molecular oxygen. The molecular weight was estimated approximately 23, 000 according to the Archbald method. The pigment was a weakly basic protein containing copper and gave a positive test for carbohydrate.
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Part IV Distribution of the Growth Factor for a Bacterium Inducing Malo-lactic Fermentation
Hajime YOSHIZUMI, Teruo AMACHI
1969 Volume 33 Issue 1 Pages
18-24
Published: 1969
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A malo-lactic fermentation (MLF) bacterium isolated from wine requires a factor contained in tomato juice for its growth. This type of the growth stimulating substance is found in several plants, particularly those belonging to fruits. The content of the factor in grapes varies depending on the varieties, degree of ripeness and harvest time. From these observation the relationship between the induction of MLF and the activity of the factor of grapes was discussed.
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Part V Purification of an Intracellular New Nuclease
Takeshi UOZUMI, Gakuzo TAMURA, Kei ARIMA
1969 Volume 33 Issue 1 Pages
25-30
Published: 1969
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An intracellular nuclease, which is known to be activated about three times in the course of autolysis of
Asp. oryzae,
1, 2) was partially purified in the present work. A portion of mycelia (20.6kg) obtained by submerged culture was autolyzed in 35 liters of water with 500ml of toluene at 30°C for 40hr. The nuclease was 18, 300 times purified from the filtrate of the autolyzate by chromatographies using CM-cellulose and Sephadex G-100. The DNase activity and the RNase activity were eluted at the same position in each chromatography, and the ratio of two activities was constant throughout the last steps of purification.
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Katuhiko NODA, Akira YOSHIDA
1969 Volume 33 Issue 1 Pages
31-35
Published: 1969
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Effects of several hormones on liver histidase activity were investigated to study the mechanism of elevation of the activity of histidase when rats were fed on a histidine imbalanced diet.
Continuous injection of thyroxine (5_??_10 μ
g/rat/day) diminished histidase activity, and hypophysectomy increased histidase activity as compared with that of non treated animals. No change was observed on alanine transaminase (GPT) by above treatments.
Neither histamine, insulin, triamcinolone, adrenaline, nor adrenalectomy affected on the activity of histidase. Injection of histidine to rats fed on a histidine imbalanced diet made the activities of histidase and GPT tend to decrease.
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Part III. Effect of Phytic Acid on Coagulative Reaction in Tofu-making
Kyoko SAIO, Emiko KOYAMA, Sadao YAMAZAKI, Tokuji WATANABE
1969 Volume 33 Issue 1 Pages
36-42
Published: 1969
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This paper constitutes the third in a research series undertaken to clarify the interactive relation among soybean meal protein, calcium and phytic acid, dealing with the practical subject closely related to tofu-making. The results may conclude that phytic acid, naturally present in solybean seeds or added to soybean milk, gave significant effect on physical properties of prepared tofu-gel. The higher content of phytic acid in soybean milk resulted in the slower coagulative reaction between soybean protein and calcium, which is favorable for tofu gel formation.
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Akira YOSHIDA, Kiyoshi ASHIDA
1969 Volume 33 Issue 1 Pages
43-49
Published: 1969
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Requirement pattern of essential amino acids for growing rats was investigated at low dietary level of nitrogen (0.62% of nitrogen) for the basic knowledge of amino acid supplementation to low protein diets. The basal diet contained 3% of essential amino acid mixture and 2% of non-essential amino acid mixture with appropriate amount of the other nutrients. The present pattern indicated relatively lower requirement for lysine and rather higher requirement for phenylalanine, valine and arginine as compared with those of several workers obtained for maximum growth of rats with diet containing sufficient amount of nitrogen. Application of dose response curves of each amino acid for the estimation of the order of the limiting amino acids was suggested.
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Effect of C10- and C15-Terpenols on Acetate-2-14C Incorporation into Ipomeanmarone
Itaro OGUNI, Kazuko OSHIMA, Hidemasa IMASEKI, Ikuzo URITANI
1969 Volume 33 Issue 1 Pages
50-62
Published: 1969
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Using sweet potato root tissue infected with
Ceratocystis fimbriata, the effect of farnesol, nerolidol or geraniol on the incorporation of acetate-2-
14C into ipomeamarone and other lipids, and on the production of ipomeamarone and other lipids was examined. Acetate-2-
14C incorpration into ipomeamarone and other lipids and the production of the lipids except ipomeamarone were markedly suppressed by the addition of the terpenol. The production of ipomeamrone was unaffected. It was concluded that the marked decrease of acetate incorporation into ipomeamarone in the presence of the terpenol was due mainly to the direct inhibition of the biosynthetic pathway and to a lesser extent to their dilution effect. The inhibitory sites by the terpenol seemed to be localized in at least 2 positions in the biosynthetic pathway of ipomeamarone. After feeding tissue disks with actetate-2-
14C in the presence of farnesol or geraniol, either farnesol or geraniol was isolated, and the respective crystalline derivatives were found to be radioactive.
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Part XII. Screening of Alicyclic Hydrocarbon-Assimilating Microorganisms and trans-4-Ethylcyclohexanol Formation from Ethylcyclohexane
Yoshiaki ARAI, Koichi YAMADA
1969 Volume 33 Issue 1 Pages
63-68
Published: 1969
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1. Five strains of bacteria were isolated from soil samples with ethylcyclohexane as the main source of carbon.
2. The strain S6BI which showed the best growth in the liquid medium was identified as Alcaligenes faecalis.
3. The main product was separated from culture broth and identified as trans-4-ethyl-cyclohexanol.
4. The mixture of trans- and cis-isomers of 4-ethylcyclohexanol was separated and submitted to the identification.
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von Ryoya NIKI, Shunrokuro ARIMA
1969 Volume 33 Issue 1 Pages
69-74
Published: 1969
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The temperature-dependent polymerisation of β-casein was investigated by the method of light scattering, ultracentrifuge and optical rotatory dispersion. β-Casein used in the experiment was fractionated into β
fast-casein and β
slow-casein according to the method of Gehrke
et al. β
slow-Casein exhibited a strong tendency to associate with rising temperature, while β
fast-casein did not show such a property. The temperature-dependent polymerisation of β
slow-casein was extremly concentration dependent. On the other hand the results of optical rotatory dispersion indicated that β-casein changed structurally with the change in temperature, and the structural changes occured may affect upon the ability of the polymerisation.
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Heterogeneity of Ovomucoid
Masao KANAMORI, Makoto KAWABATA
1969 Volume 33 Issue 1 Pages
75-79
Published: 1969
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The carbohydrate of ovomucoid was analyzed for components I, II, III and IV which were. fractionated by CMC-column chromatography. The total hexose content and the molar ratio of D-mannose to D-galactose (4:1) were identical in each component, but the D-glucosamine and sialic acid contents were found to be higher in components I and II (both are trypsin inhibitors) compared with components III and IV (both are apo-proteins of flavomucoid). The amino acid composition of each component of ovomucoid varied considerably. There were remarkable differences in the amino acid composition between components I and II, both had an antitryptic activity. The N-terminal amino acid of components I and II was alanine and in the case of components III and IV, threonine was found on the N-terminal. The free carboxylic residue of sialic acid was found to be responsible for the negative charge of ovomucoid, and its electrophoretic heterogeneity was reaffirmed by paper electrophoresis. It is evident from the ultracentrifugal analysis that the four components of ovomucoid have a similar molecular size.
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Atuko KIKKAWA, Hiroshi MEGURO, Katura TUZIMURA
1969 Volume 33 Issue 1 Pages
80-85
Published: 1969
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ORD and CD of D (+) pantothenic acid, D (+) pantothenyl alcohol and D (-) pantolactone were studied. The acid and the alcohol gave positive Cotton effects with a peak at 227 and 225mμ, respectively, and the lactone gave a negative Cotton effect with a peak at 227 and 225mμ, respectively, and the lactone gave a negative Cotton effect with a trough at 233mμ. They gave CD maxima at 214, 213 and 219mμ corresponding to the inflection points of their ORD curves.
The concentrations were found to be linear to the rotation angles and the possibility of the application to quantitative analysis of the ORD was cited. The ORD showed the quantitative formation of the lactone by acid treatment of the vitamins without any racemization and hence the determination via lactone was suggested.
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Part XII. On the Reduction of (-)-Perillaldehyde
Hiroshi KAYAHARA, Hiroo UEDA, Ken'ichi TAKEO, Chuji TATSUMI
1969 Volume 33 Issue 1 Pages
86-89
Published: 1969
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The reduction of (-)-perillaldehyde (I) with sodium in aqueous ammonia afforded mainly
trans-and
cis-dihydroperillylalcohols (III
a and III
b). This fact indicates that this reduction method can surely be applied to α, β-unsaturated monoterpene aldehydes which are extremely sensitive to alkali and that the unconjugated carbon-carbon double bond is normally inert to this reducing condition.
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Shimpachi KONISHI, Katsusuke KAGEYAMA, Teruo SHIRO
1969 Volume 33 Issue 1 Pages
90-95
Published: 1969
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One of adenine-riboflavin doubleless mutants derived from
Bacillus subtilis strain AJ 1987, an adenine requiring and inosine producing strain, was found to accumulate a fluorescent material in the culture medium. This material was identified as 6, 7-dimethyl-8-ribityl-lumazine (LUM). Several culture conditions for LUM formation were studied. In general, accumulation of LUM reached approximately 90mg per 100-ml medium in 3 days.
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Part III. 3-Phenoxypyridazines and Related Compounds
Teruomi JOJIMA, Nariyoshi YOSHIMURA, Tetsuo TAKEMATSU, Saburo TAMURA
1969 Volume 33 Issue 1 Pages
96-102
Published: 1969
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3-Phenoxypyridazines and related compounds were evaluated their pre-emergenceactivities. Of these, 3-phenoxy-, 3-(2-methylphenoxy)-, and 3-(2-ethylphenoxy)-pyridazines (III, IV and VII) showed powerful effects on barnyardgrass and spikerush, whereas they gave no injury on rice plants. Furthermore, susceptibility of various kinds of cultivated plants as well as of weeds to III and IV were examined. Growth regulating activity of III was compared with that of maleic hydrazide.
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Part V. Isolation of Trisaccharides Containing α-1, 3 Linkage from a Dextran of Leuconostoc Mesenteroides B
Fumio YAMAUCHI, Kazuo MATSUDA
1969 Volume 33 Issue 1 Pages
103-109
Published: 1969
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Fragmentation of a dextran from
Leuconostoc mesenteroides B
* by controlled acetolysis gave five trisaccharides; isomaltotriose (0.12%), 3-α-isomaltosylglucose (0.80%), 6-α-nigerosylglucose (0.43%), 3, 6-di-α-glucosylglucose (0.18%) and nigerotriose (0.12%). Among these trisaccharides the last three were first isolated from dextran. Isolation of nigerotriose indicated that some of the 1, 3-linkages in the dextran molecule were contiguous.
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Part II. A Novel Cyclization to Dehydrorotenoids
Nobuji NAKATANI, Masanao MATSUI
1969 Volume 33 Issue 1 Pages
110-112
Published: 1969
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A general cyclization reaction to dehydrorotenoids has been developed. Dehydrorotenone (III) and dehydrodeguelin (IV) were obtained in high yield from methyl derrisate (Vb) and methyl deguelate (VIb), respectively, by treatment with sodium ethoxide.
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Ryoji ONODERA, Makoto KANDATSU
1969 Volume 33 Issue 1 Pages
113-115
Published: 1969
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Chitoshi HATANAKA, Junjiro OZAWA
1969 Volume 33 Issue 1 Pages
116-118
Published: 1969
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Bunji SHIMIZU, Akio SAITO
1969 Volume 33 Issue 1 Pages
119-121
Published: 1969
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Isao TAKEDA
1969 Volume 33 Issue 1 Pages
122-124
Published: 1969
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Jun-Ichiro MUKAI, Jun INOUYE, Satoru AKUNE
1969 Volume 33 Issue 1 Pages
125-127
Published: 1969
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Kensuke FURUKAWA, Tomoo SUZUKI, Kenzo TONOMURA
1969 Volume 33 Issue 1 Pages
128-130
Published: 1969
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Yuichi HATSUDA, Takashi HAMASAKI, Masayoshi ISHIDA, Seikichi YOSHIKAWA
1969 Volume 33 Issue 1 Pages
131-133
Published: 1969
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Michio MATSUHASHI, Ikuko OHARA, Yoshihiro YOSHIYAMA
1969 Volume 33 Issue 1 Pages
134-137
Published: 1969
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