Abstract
The presence of psicofuranine in the fermentation meduim caused the accumulation of a copious amount of 5'-XMP by brevibacterium ammoniagenes. The accumulation of 5'-XMP in the meduium was considered to be due to the inhibition of converting 5'-XMP to 5'-GMP by psicofuranine, which is known as a specific inhibitor of XMP aminase.
It was previously repored that in 5'-IMP fermentation with Br. ammoniagenes pantothenate and thiamine, in addition to biotin which was required for the growth of the microogrganism, were exclusively required. This requirement for both vitamins was also observed in 5'-XMP production induced by thed antibiotic.
The addition of manganese in excess to the fermentation medim promoted the bacterial growth greatly and inhibitedd IMP production, whereas XMP Production induced by piscofuranine wa not affected by the addition of excess manganese.
The accumulation of XMP induced by the antibiotic was completely suppressed by the presence fo purine derivatives such as guanine, and xanthine deriatives, and partially by hypoxanthine.
5'-XMP was identified by chemical and enzymatic analyse and UV absorption spectrum.
