Studies on Transglycosidation to Vitamin B₆ by Microorganisms

Part IV. Purification of a Bacterial Enzyme Catalyzing Pyridoxine Glucoside Synthesis

Abstract

The enzyme activity to synthesize pyridoxine glucoside was demonstrated in intact cells and cell extracts of genera, Sarcina and Micrococcus. The isolated and identified strain, Micrococcus sp. No. 431 was found to have high activity of this enzyme in its cell extract. The enzyme activity reached to a maximum after 20 hr of cultivation.
The enzyme which synthesized pyridoxine glucoside via transglucosidation from sucrose to pyridoxine was purified from Micrococcus sp. No. 431 by means of ammonium sulfate fractionation, DEAE-Sephadex, hydroxylapatite and Sephadex G-100 column chromatogra-phies. The enzyme was purified about 354-fold and confirmed to be homogenous in poly-acrylamide-gel electrophoresis and ultracentrifugation.