Isolation of Alkaline Protease Inhibitor Producing Microorganisms

Abstract

For the purpose of obtaining microorganisms capable of producing alkaline protease inhibitor, screening test was carried out. One strain of microorganisms (No. S-3253) showed strong ability to produce alkaline protease inhibitor.
The morphological and physiological characteristics of strain S-3253 were studied. This strain was found to belong to Streptomyces albogriseolus.
When the strain S-3253 was cultivated at 45°C with a medium containing 4% polypepton, 2% starch, 0.1% yeast extract, 0.1% NaCl, 0.1% K₂HPO₄ and 0.05% MgSO₄-7H₂O in shake-flasks (pH 7.0), the highest activity was obtained after 40_??_48 hr cultivation.