1972 年 36 巻 8 号 p. 1405-1412
Brevibacterium ammoniagenes ATCC 6872 was previously reported to accumulate large amounts of IMP, AMP, ADP, ATP, GMP, GDP and GTP from the corresponding purine bases. The organism was also reported to convert various derivatives of purine and 8-azapurine to the corresponding ribotides.
Using the similar process, ribotidation of pyrazolo [3, 4-d] pyrimidines was attempted, and it was found that the same organism was able to produce remarkable amounts of 4-hydroxy-1-β-D-ribofuranosylpyrazolo [3, 4-d] pyrimidine 5'-monophosphate (HPP-RP) from 4-hydroxy-pyrazolo [3, 4-d] pyrimidine (HPP, allopurinol) and 4-amino-l-β-D-ribofuranosylpyrazolo [3, 4-d] pyrimidine 5'-monophosphate and 5'-diphosphate from 4-amino-pyrazolo [3, 4-d] pyrimidine.
The crystals of HPP-RP (Na-salt) were isolated from the cultured broth of Br. ammoniagenes incubated with HPP, and characterized based on UV-spectra, IR-spectrum, NMR and others.
It was also found that HPP-RP was converted to the corresponding riboside by hydrolysis in aqueous solution (pH 4.0_??_9.0) for 6 hr at 140°C. The hydrolysis of HPP-RP was also accomplished with various organisms.
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