Microbial Synthesis of GDP-glucose

抄録

GDP-glucose pyrophosphorylase activity was found to be widely distributed in Streptomyces. Streptomyces olivaceus IFO 3409 showed the highest activity among the strains tested. When air-dried cells of Streptomyces olivaceus (100mg/ml) were incubated with GTP•(NH₄)₃ (20μmoles/ml), D-glucose-l-phosphate (100μmoles/ml), potassium phosphate buffer, pH 7.0 (270μmoles/ml) and MgSO₄ (20μmoles/ml), GDP-glucose was synthesized in 25% yield against GTP. GDP-glucose was isolated and identified. The strains capable of synthesizing GDP-glucose in higher yield were searched. Streptomyces sp. AKU 2801 seems to be the most suitable strain for the preparation of GDP-glucose.