γ-Polyglutamic Acid Depolymerase Induced by Infections of natto and subtilis Phages and Its Further Properties
1973 年 37 巻 1 号 p. 83-90
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1973 年 37 巻 1 号 p. 83-90
The known B. subtilis phages PBSI, M2, SP 01, SP 10 and newly isolated B. natto phage NP-38 were examined for the induction of γ-polyglutamic acid (PGA) depolymerase synthesis. These phages, except M2, were capable of inducing the synthesis of the depolymerase in the infected bacteria. A direct evidence that the synthesis of depolymerase lay under the phage genome was obtained by the isolation of a depolymerase-deficient mutant of phage. A measurable amount of depolymerase was not detected in either intact or disrupted particles of phages. Thus, it seemed that the phage particles did not involve a bound enzyme.
The depolymerase was purified about 960 folds from the lysate of phage NP-38. The purified enzyme did not have a hydrolytic action on known protein substrate. The molecular weight was estimated to be about 20, 000 from the result of a gel filtration.
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