Utilization of n-Paraffins and Vitamin B₆ Production by Pichia guilliermondii Wickerham

Abstract

The accumulation of vitamin B₆ by Pichia guilliermondii Wickerham NK-2 strain grown on hydrocarbon was investigated. Ammonium acetate was more effective than other nitrogen sources tested. Satisfactory utilization by the yeast strain was observed in n-alkanes of C₁₀-C₁₈, and n-pentadecane was the best for vitamin B₆ production. Vitamin B₆ was excreted in the cultural broth mainly in the form of pyridoxal. The maximal vitamin B₆ production was approximately 25mg per liter of the culture broth.