An Improved Method for the Fermentative Production of Coenzyme A from Pantothenic Acid, Cysteine, and 5'-AMP

Abstract

The cultivation of Brevibacterimn ammoniagenes IFO 12071 with pantothenic acid, cysteine, and 5'-adenylic acid gave coenzyme A in a high yield. The organism was stabilized by repeated single colony isolations. The culture conditions optimal for the production of coenzyme A were investigated, and the yield of coenzyme A in the culture broth reached more than 3mg/ml.
The advantages and disadvantages of the present method were discussed by comparing them with our original dried cell method.