Abstract
The accumulation of NAD was studied by culturing yeast in the presence of NAD precursors. Among the strains tested, Saccharomyces carlsbergensis showed the highest ability for the accumulation of NAD. Additions of pantothenate, inositol, zinc ion and fatty acids were effective for the accumulation of NAD. Under the optimal culture condition, NAD level in Saceharomyces carlsbergensis reached 42mg per gram dry cells. Surfactants belonging to alkyl sulfate were effective on the leaking of the intracellular NAD, and about 75mg of NAD per 100ml was accumulated.
