Abstract
A study was made to determine a method for the production of NAD using baker's yeast, and a suitable secondary culture condition for the accumulation of NAD was established. From the study the following results were obtained: Nicotinamide, nicotinic acid and adenine were effective on the accumulation of NAD. However, ribose or tryptophan-one of the precursor of NAD-was not effective. NaF, KCN or NaN3-metabolic inhibitors-inhibited the accumulation of NAD. Baker's yeast obtained from commercial source was cultured secondarily in the medium containing 0.3% adenine, 0.6% nicotinamide in 0.2M K2HPO4 (50% fresh yeast was added), pH 4.5. Under this optimal condition, NAD content reached about 12mg/g dry cells (corresponding to 2.0mg/ml medium), and it corresponded to about 20 times that of the initial content.
