Abstract
Incorporation of 14C from various 14C-labelled compounds into vitamin B6 (abbreviate as B6) in a cell-suspension of B6-producing bacteria, Achromobacter cycloclastes A. M. S. 6021, was studied by using a newly designed purification procedure of the radioactive B6. The procedure consisted of Sephadex G-25 gel filtration, Dowex 50W-X8 column chromatography, Amberlite CG-50 column adsorption, powdered cellulose partition column chromatography, crystallization and sublimatography. It was observed that the labels both from 1, 3- and from 2-labelled glycerol were clearly incorporated into B6 and the label of 14C-labelled γ-aminobutyric acid was also incorporated. The incorporation of 14C from 14C-labelled glycerol or γ-aminobutyric acid was affected by the addition of cold γ-aminobutyric acid or glycerol. The implication of these compounds as precursors of B6 was discussed.
