抄録
An ultracentrifugally homogeneous preparation of the cyclodextrin glycosyltransferase was obtained from the culture broth of a strain of Bacillus species (No. 5 strain) by a purification methods including adsorption on corn starch, ammonium sulfate precipitation, chromatography on DEAF-cellulose column, and gel-filtration on Sephadex G-100. This enzyme preparation (No. 5 enzyme) was separated into two fractions in disc-electrophoresis and Ampholine-electrophoresis (Fr. 1: pI 6.07 and Fr. 2: pI 6.80) which had the ability to form cyclodextrin. These two fractions were similar to each other in the enzymic properties except the isoelectric point. These enzymes differ from the amylase from Bacillus macerans (BMA) in the enzymic properties. Furthermore, No. 5 enzymes differ from BMA in the action on starch. The authors consider that No. 5 enzymes mainly produce the cycloheptaamylose from starch and on the contrary, BMA mainly produces the cyclohexaamylose from starch.
