Abstract
An extracellular acidic polysaccharide produced by Serratia piscatorum IFO 12527 was found to exhibit a marked anti inflammatory activity. The polysaccharide was purified by fractional precipitation with cetyltrimethylammonium bromide and then by gel filtration on Sepharose 2B to give two homogeneous fractions, PLS N-I and PLS N-II, the former exhibiting the anti inflammatory activity.
PLS N-I was a complex polysaccharide composed of L-rhamnose, D-galactose and D-galacturonic acid in the molar ratio of 2:1:1, together with small portions of D-glucosamine, D-galaetosamine, protein and fatty acids such as acetic, lauric, myristic, β-hydroxymyristic and palmitic acids. Physicochemical and biological properties of PLS N-I and PLS N-II were also described.
